The Science Journal of the American Association for Respiratory Care

1999 OPEN FORUM Abstracts

ASSESSMENT OF POLYMERASE CHAIN REACTION AND SEROLOGY FOR DETECTION OF CHLAMYDIA PNEUMONIAE IN THE PATIENTS WITH ACUTE RESPIRATORY TRACT INFECTION.

SHI Yi, XIA Xirong, SONG Yong, KANG Xiaoming, FENG Genbao, HU Lanping. Department of Respirology, Jinling Hospital, Medical college of Nanjing University, Nanjing, 210002, P.R. China.

Objective: To find out the infection rate and the clinical features of patients with acute respiratory infection caused by Chlamydia pneumoniae (C. Pneumoniae). Method: A prospective study for C.pneumoniae infection was conducted in 110 patients with respiratory tract infection from January-December 1995 in Nanjing. Sputum and throat swab specimens were taken and C. pneumoniae DNA were detected by polymerase chain reaction (PCR) with the HM-1-HR -1 primer pair. At the same time, serum samples were taken and immunoglobulins G and M (IgG and IgM) fractions of antibodies to C. pneumoniae were studied by microimmuno-fluorescence test.

Results: Prevalence of specific IgG was 70% in patients with respiratory tract infection. Seventeen patients (16%) were serologically diagnosed as having recent C. pneumoniae infection and 12 patients (11%) had the positive PCR in sputum and/or swab specimens. The total positive rate was 23% (25/110) combining PCR with serology. Acute infection of C. pneumoniae was common in the patients with asthma (57%), pneumonia (35%), COPD (26%) and bronchitis (25%). The clinical feature between C. pneumoniae infection and non-C.pneumoniae infection was that there was no significant difference. Conclusion: Our data suggest that Chlamydia pneumonia is an important pathogen that causes infections of the human respiratory tract and attention should be drawn to this special illness.

OF-99-241

You are here: RCJournal.com » Past OPEN FORUM Abstracts » 1999 Abstracts » ASSESSMENT OF POLYMERASE CHAIN REACTION AND SEROLOGY FOR DETECTION OF CHLAMYDIA PNEUMONIAE IN THE PATIENTS WITH ACUTE RESPIRATORY TRACT INFECTION.