The Science Journal of the American Association for Respiratory Care

2007 OPEN FORUM Abstracts

MAST CELL STABILIZING ACTIVITY OF GLYCYRRHIZA GLABRA LINN.

G. P. Choudhary1, S. C. Chaturvedi1, S. Bharti1


Background: The mechanism of the inflammatory response resulting in asthma is complex and involves numerous cell types, including mast cells. The mast cell has long been associated with asthma, since it releases a variety of preformed and newly synthesized mediators that could account for several features of asthma. Among the mediators released from mast cells, histamine is a well-characterized and the most potent vasoactive mediator in acute bronchoconstriction.In the present study the ethanolic extract of roots glycyrrhiza glabra was screened for mast cell degranulation properties.

Methods:Albino rats were sensitised by horse serum along with triple antigen containing Bordetella pertussis organism .Treatment of drug extract and standard given for 14 days ,on the 14th day 3h after the last dose treatment rats were sacrificed and intestinal mesentery was taken for study of mast cell.
Aims of the study: The use of herbal medicines is based on traditional healing, and is also influenced by culture. In India, the use of medicinal plants and herbal therapy has been practised long before recorded history.
However, scientific knowledge concerning the uses of medicinal plants in asthmatic disease is very limited. It is likely that some of the plants used have no significant effect on respiratory disorders. In this study, we investigated glycyrrhiza glabra which is traditionally used in India for asthma therapy, and focused our attention on their inhibitory action against histamine release from mast cell.

Results: In the unsensitized rats, 12.55±1.8% of the mast cells were found to be in the process of degranulation. In the sensitized untreated rats, 80.90±4.65% of the mast cells were degranulating when challenged with the antigen. At the doses used, prednisolone as reference standard were found to inhibit degranulation of mast cells to an extent of 72.25±3.91%, respectively. The ethanolic extract at200 and 400 mg/kg body weight was found to inhibit degranulation of mast cells to an extent of 59.25±2.96and 67.34±3.50%, respectively.
All the values obtained were highly significant (P<0.001,Table 1).

Conclusion:The effect of ethanolic extract at two different dose level(200mg/kg and400mg/kg),markedly protected the rats against antigen induced challenge of mast cell. Preliminary phytochemical analysis showed positive response for flavonoids. Flavonoids are reported as mast cell stabilizer active principle of plant origin.

Table 1-Mast cell stabilizing activity of ethanolic extract of Glycyrrhiza glabra.
Group Treatment Dose(mg/kg body weight Route of administration Granulated mast cells(%)(mean ±S.E.) Degranulated mast cells(%)(mean ±S.E.)
I Control(Tween 80,1%)* _ Oral 87.44±4.62 12.55±1.80
II Control(Tween 80,1%) _ Oral 21.32±1.78 80.90±4.65
III Prednisolone 10 Oral 72.25±3.91* 28.14±1.34*
IV Ethanolic extract 200 Oral 59.25±2.96* 41.32±2.70*
V Ethanolic extract 400 Oral 67.34±3.50* 33.68±2.11*

*Not treated with horse serum and triple antigen.

Values are mean ± S.E.
n=6
*P<0.001(Students t-test)

You are here: RCJournal.com » Past OPEN FORUM Abstracts » 2007 Abstracts » MAST CELL STABILIZING ACTIVITY OF GLYCYRRHIZA GLABRA LINN.