The Science Journal of the American Association for Respiratory Care

2009 OPEN FORUM Abstracts

GLUTAMINE INHALATION ATTENUATES ENDOTOXIN-INDUCED LUNG INJURY IN RATS: ROLE OF ENHANCED HEAT SHOCK PROTEIN 72

Yvonne Y. Juang1, Chiao-Wen Tu1, Huei-Ping Dong2, Rei-Cheng Yang1, Ming-Shyan Huang1, Tung-Heng Wang1; 1Department of respiratory Therapy, Kaohsiung medical university, Kaohsiung, Taiwan; 2Foo-Yin University, Kaohsiung, Taiwan

Background: Studies have demonstrated that heat shock proteins (HSPs) play an important role in the protection of stressed organisms. The development of strategies for enhancing HSPs expression may provide novel means of minimizing inflammatory lung condition, such as acute lung injury. This study aimed to examine that glutanmine (L-alanyl-L-glutamine) inhalation may enhance pulmonary HSP expression and attenuate lung damage in a model of acute lung injury induced by Lipopolysaccharide (LPS) inhalation. Methods: Male Sprague-Dawley rats weighing 280-380g were randomly assigned to four experimental groups, Group 1:saline inhalation; Group 2:glutamine inhalation (1.5g); Group 3: pretreatment of saline inhalation 12 hours before LPS inhalation (2mg); Group 4: pretreatment of glutamine inhalation (1.5g) 12 hours before LPS inhalation(2mg). Samples were obtained 12 hours after treatment in group 1 and 2, and 8 hours after LPS inhalation in group 3 and 4. Nebulization of inhaled agents was performed by Aeroneb Pro (AERO). The degree of lung injury was assessed by various parameters including pathologic change in lung tissue, and protein concentration, lactate dehydrogenase(LDH) activity, total cells and polymorphonuclear leukocyte (PMN) cell counts in the bronchoalveolar lavage fluid (BALF). HSP 72 was examined by Western blot analysis. The data are expressed as mean±SEM. Statistical differences among groups were determined using paired Student t test. Results: Glutamine, compared with saline administration, led to significant increase in lung HSP 72 both in non LPS-treated rats (group 1 compared with group 2, P≤0.002 ) and LPS-treated rats (group 3 compared with group 4, P≤0.05 ). In LPS-treated rats, pretreatment of glutamine inhalation exhibited less lung injury by the evidences of decrease in histological damage score (11.4±1.5 Vs. 7.0±1.7, P≤0.001) and dramatic decrease protein concentration (2.61±0.63 Vs. 1.02±0.35mg/mL, P≤0.05), LDH activity ( 129.2±24.5 Vs.78±14.48 mAbs/min, P≤0.001) and differential cell counts,PMN % (88.6±7.3 Vs. 52.3±17.6%, P≤0.001) in the BALF. Conclusion: The present results indicate that previous glutamine inhalation associated with the enhancement of HSP 72 synthesis attenuates tissue damage in experimental lung injury. Sponsored Research - None

You are here: RCJournal.com » Past OPEN FORUM Abstracts » 2009 Abstracts » GLUTAMINE INHALATION ATTENUATES ENDOTOXIN-INDUCED LUNG INJURY IN RATS: ROLE OF ENHANCED HEAT SHOCK PROTEIN 72