2010 OPEN FORUM Abstracts
PROTEIN KINASE ACTIVITY AND EXPRESSION OF ITS ISOENZYMES DURING THE ONSET OF ASTHMA.
Rakesh K. Mishra; biochemistry&molecular biology, universsity of texas medical branch, Galveston, TX
Protein Kinase C (PKC) plays a pivotal role in airway inflammation in asthma. However, changes in PKC activity and expression at the initiation of airway inflammation need to be studied in relation with airway hyper-reactivity. In this study, we have used the male guinea pig were sensitized with ovalbumin. Airway inflammation was checked by intradermal test and airway functions (after ovalbumin challenge) assessed at 30 min on day 0, 9 and 14. Blood and lung specimens were obtained for lymphocytes, airway smooth muscles (ASM) and histopathological examination. PKC activity and its expression were determined in ASM and lymphocytes. Day 9 was the day of initiation of airway inflammation. In OVA sensitized group PKC activity and its expression (PKCa and PKCe) increased both in ASM and lymphocytes on day 9 at 30 min which remained almost unchanged up to day 14.Histological examination of the lung revealed mild peribronchial chronic lymphocytic inflammation on day 9 at 30 minutes. On day 14, moderate infiltration of lymphocytes and eosinophils was seen at 30 minutes which progressively increased during late hours. The results of this study suggest that PKC signal transduction pathway participates in the regulation of the activation of lymphocytes and ASM and its activation leads to the initiation of airway inflammation in animal model, which are the characteristic features of asthma. Sponsored Research - None