The Science Journal of the American Association for Respiratory Care

2010 OPEN FORUM Abstracts


Alyssa Bistak, Melissa Frasure, Jilliann Hoyt, Dana Khy, Leah Seebon, F. Herbert Douce; Respiratory Therapy, The Ohio State University, Columbus, OH

BACKGROUND: Arterial blood gases (ABG) play a vital role in determining respiratory care. Previous research has shown unstable ABG values stored in some plastic samplers for 30 minutes and at cold temperatures. The purpose of this study was to compare two brands of plastic arterial samplers and a glass control over time and at a cold temperature using a Cold Specimen Transporter (CST) to determine if the plastic samplers keep the blood gases as stable as blood stored in glass. METHODS: We created arterialized human blood using an extracorporeal circuit at 37oC with 12% O2 and 5% CO2. We tested Radiometer Safe Pico Self-fill, Smiths Medical Portex® Line Draw and Roche microsamplers. Using a Rapidlab® 1200, samples were analyzed for PO2 and PCO2 at 0, 15 and 30 minutes; at 22oC or 7-8oC using a CST. To identify statistically significant differences between each brand of sampler, temperature and storage time, we used ANOVA with repeated measures and Tukey’s HSD to compare the PO2 and PCO2 values. We considered differences statistically significant when p < 0.05. RESULTS: For PO2 values, there were no statistically significant differences among the samplers at 0 or 15 minutes or the Portex sampler combined with a CST at 0, 15 or 30 minutes; whereas at 30 minutes, Portex and Pico samplers were significantly different from the Roche control sampler at 0 and 15 minutes. The PCO2 values were significantly different in Portex, Pico, and Portex CST samplers when compared to control under multiple conditions. The average change in our PO2 values at 30 minutes was up to 12 mm Hg and was considered clinically important; whereas, the average change in PCO2 values was 2 mm Hg and was not considered important. CONCLUSIONS: Our results are similar to other studies for blood stored for 30 minutes. Storage time of arterial blood should not be prolonged; ABG analysis should occur within 15 minutes. There are no significant differences in stability between the plastic samplers we tested. The protective properties of the CST should be further investigated. Sponsored Research - None Means (standard deviations) of PO2 values of arterialized blood stored in different samplers under variable times and temperatures