The Science Journal of the American Association for Respiratory Care

2011 OPEN FORUM Abstracts

COMPARISON OF METHODS FOR MEASUREMENT OF BLOOD GASES AND WHOLE BLOOD LACTATE LEVELS BETWEEN THE OPTI-MEDICAL CCA-TS PORTABLE BLOOD GAS ANALYZER AND THE ROCHE COBAS B 221 BENCHTOP ANALYZER.

Daniel J. Grady, Terrence Smith; Respiratory Care, Mission Health System, Asheville, NC

Background: Blood lactate measurements and hyperlactatemia may be utilized as indicators of tissue hypoxia, mitochondrial disease, and acute lung injury. In addition, blood lactate has been found to significantly correlate with Oxygen delivery per minute. Increasingly, blood lactate measurements are available in blood gas analyzers. The purpose of this study was to evaluate the performance of the pH, PCO2, PO2, Lactate, tHb, and SO2 sensors of the OPTI CCA-TS blood gas system and to correlate whole blood measurements with the Roche Cobas b 221 benchtop analyzer. The project was reviewed and received exemption by the Institutional Review Board. Methods: Two standard reference cassettes were run once per day on two Opti Medical analyzers used in the study for daily quality control. In addition, three aqueous controls (Opti check level 1, 2, and 3 were run in duplicate on each day of the study on the same B-Lac cassettes used to collect the method comparison. A total of 52 (n = 52 ) heparinized, whole blood, patient samples were utilized for analysis in the Opti Medical analyzers immediately following blood gas analysis in the Roche Cobas b 221 analyzer. Samples were de-identified to prevent traceability to the patient. A sequence number was assigned to the sample to facilitate correlation between the OPTI CCA-TS results and the Cobas measurement. Each sample was tested in duplicate on the B-Lac cassette in the OPTI CCA-TS analyzer and compared against a single measure on the Cobas analyzer. Results: Regression analysis and Pearson's product-moment correlation were calculated for the pH, pCO2, PO2, Lactate, tHb, and SO2 results measured with the Cobas b 221 and OPTI CCA-TS system. Correlation results are summarized in the table below. Conclusions: The Opti Medical CCA-TS portable blood gas analyzer utilizes a principle of optical fluoresence and operates via disposable cassettes for blood gas and lactate measures; rather than using conventional blood gas electrodes. We conclude that the OPTI Medical CCA-TS system using the principle of optical fluorescence provided strong correlations with the the Cobas b221 blood gas analyzer for whole blood measures of ph, pCO2, pO2, SO2, Lactate, and tHB.
Sponsored Research - The study was sponsored by Opti-Medical.
Correlations between the Opti CCA-TS and the Cobas b221 Blood Gas Analyzers