2011 OPEN FORUM Abstracts
MEASUREMENT OF IN VITRO CHANGES IN ARTERIAL BLOOD GASES FOLLOWING INFUSION OF SUPERSATURATED DISSOLVED OXYGEN SOLUTION.
Daniel J. Grady1, Terrence F. Smith1, Michael A. Gentile2; 1Respiratory Care, Mission Health System, Asheville, NC; 2Department of Pulmonary and Critical Care Medicine, Duke University Medical Center, Durham, NC
Background: Patients experiencing acute myocardial infarction; who undergo PCI and reperfusion with solutions containing dissolved pO2 tensions of 760-1000 mm. Hg infused directly into coronary arteries, achieve significant reductions in myocardial infarct size 1. The purpose of this study was to evaluate the In Vitro changes in arterial blood gases following injection of minute volumes of supersaturated dissolved oxygen solutions in discarded arterial blood samples. Methods: Using a novel hyperbaric tonometer, sterile water was supersaturated with 100% Oxygen gas by bubbling a gas flowrate of 10L/min into 500 ml. of sterile water exposed to a 17 degree C water bath for 20 minutes; at ambient barometric pressure (Pb = 763 mm. Hg.). The supersaturated oxygen solution produced a mean dissolved pO2 of 980 mm. Hg at 17 degrees C. The supersaturated dissolved oxygen solution was carefully withdrawn from the hyperbaric tonometer to avoid contact with ambient air bubbles using a tuberculin syringe. A total of 8(n = 8) discarded blood gas samples were randomly selected for baseline and repeat analysis following injection of supersaturated oxygen solution. A small volume (0.1 ml) of supersaturated, dissolved oxygen solution was directly injected into the discarded (2.0 ml) arterial blood samples, mixed, and immediately re-analyzed. All discarded samples were de-identified prior to mixing with supersaturated oxygen solution and repeat analysis. Blood gas analysis was performed using a Corning 178 Blood Gas analyzer operating at Pb = 763 mm. Hg and an electrode temperature of 37 degrees C. Results: The baseline mean pO2 of the blood gas samples was 82.1 mm. Hg. prior to injection of supersaturated dissolved oxygen solution. When 0.1 mL. of superoxygenated solution (having a mean pO2 =980 mm Hg) was injected into the discarded arterial blood gas samples, the final pO2 of the mixed blood increased to a mean of 120.0 mm. Hg, (n =8). A student "t" test indicated statistically significant differences between the baseline pO2 and mixed pO2 following injection of the superoxygenated liquid into arterial blood (p = 0.04). Results are shown in the table below. Conclusions: A supersaturated oxygen solution may be injected into arterial blood and achieve significant increases in dissolved paO2 and SaO2 In vitro. Additional research is necessary to determine clinical applications of this technology.
Sponsored Research - None
In Vitro Blood Gas Changes Following Micro-Injection of Supersaturated Dissolved Oxygen Solution
1. Stone, et al. Effect of Supersaturated Oxygen Delivery on Infarct Size After Percutaneous Coronary Intervention in Acute Myocardial Infarction. Circulation, American Heart Association, October, 2009, pp 366-374.