2011 OPEN FORUM Abstracts
EXHALED BREATH CONDENSATE ASSESSMENT IN STREET TRAFFIC-OPERATOR AND OFFICE-WORKERS EXPOSED TO SAO PAULO CITY AIR POLLUTION.
Naomi K. Nakagawa1,2, Thamires M. Lima2,1, Cristiane M. Kazama1,2, Danielle M. Goto1,2, Mariangela Macchione2, Andreas R. Koczulla3, Pieter S. Hiemstra4, Ana Luisa G. Fernandes7, Maria Lucia Bueno-Garcia6, Ubiratan P. Santos5, Paulo Hilario N. Saldiva2; 1Physiotherapy, Communication Science and Disorders, and Occupational Therapy, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, Brazil; 2Pathology (LIM05), Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, Brazil; 3Department of Pneumology, Philipps University, Marburg, Germany; 4Department of Pulmonology, Leiden University Medical Center, Leiden, Netherlands; 5Pneumology Division, Heart Institute, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, Brazil; 6Department of Internal Medicine (LIM20), Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, Brazil; 7Department of Pneumology, Escola de Medicina da Universidade Federal de Sao Paulo, Sao Paulo, Brazil
ABSTRACT Objective: Exhaled breath condensate (EBC) and nasal lavage (NL) have been used to assess biomarkers of airway inflammation. The aim of this cross-sectional study was to determine pH and cytokines in EBC and in NL of non-smoking male street traffic-operators and office-workers in Sao Paulo city. Methods: EBC and NL samples were obtained from 73 street traffic-operators (27-56 years) and 14 healthy office-workers (21-42 years). Clinical data, exhaled carbon monoxide, pH and cytokines were evaluated in EBC and NL of both groups. PM10 concentrations 8-hrs/5 days at fixed-sites were also determined. Results were analyzed by Mann-Whitney test. Results: Traffic-operators were older than office-workers (42 ± 7 and 30 ± 5 years, p < 0.001). Clinical data were similar between traffic-operators and office-workers: systolic blood pressure (118 ± 13 and 118 ± 12 mmHg, respectively), diastolic blood pressure (82 ± 8 and 78 ± 11 mmHg, respectively), heart rate (69 ± 9 and 70 ± 10 bpm, respectively) and respiratory rate (15 ±> 3 and 14 ± 2 rpm, respectively). Compared with office-workers, traffic-operators had lower EBC pH (8.12 ± 0.14 and 7.80 ± 0.46, respectively, p < 0.001) and NL pH (7.99 ± 0.33 and 7.30 ± 0.28, respectively, p < 0.001) and increased exhaled carbon monoxide (2.4 ± 0.9 and 4.5 ± 1.5 ppm, respectively, p < 0.001). The EBC acidification was combined with increased concentration of interleukin (IL)-1b and IL-10 (7-fold and 1.7 fold, respectively, p < 0.047). Cytokines in NL were similar between groups. Outdoor concentrations of PM10 was significantly higher in the work place of traffic-operators compared with the office-workers (26.67 ± 5.50 ppm and 22.53 ± 5.15 ppm, respectively, p=0.006). Conclusion: This study indicates that street traffic-operators may be under increased risk of airway inflammation due to exposure to increased air pollution in their work-day. Sponsored Research - None Demographic and clinical data of traffic-operators and office-workers