2012 OPEN FORUM Abstracts
MAST CELL STABILIZING ACTIVITY OF ETHANOLIC EXTRACT OF PIPER LONGUM LINN.
Gajendra P. Choudhary, Anupama Parate; Pharmacognosy, School of Pharmacy, Indore, India
Background: The use of herbal medicines is based on traditional healing, and is also influenced by culture. In India, the use of medicinal plants and herbal therapy has been practiced long before recorded history.However, scientific knowledge concerning the uses of medicinal plants in asthmatic disease is very limited. It is likely that some of the plants used have no significant effect on respiratory disorders. In this study, I investigated piper longum, which is traditionally used in India for asthma therapy, and focused our attention on their inhibitory action against histamine release from mast cell. Methodology: The mast cell has long been associated with asthma, since it releases a variety of preformed and newly synthesized mediators that could account for several features of asthma. Among the mediators released from mast cells, histamine is a well characterized and the most potent vasoactive mediator in acute bronchoconstriction. In the present study the ethanolic extract of fruits of piper longum was screened for mast cell degranulation properties. Albino rats were sensitized by horse serum along with triple antigen containing Bordetella pertussis organism. Treatment of drug extract and standard given for 14 days, on the 14th day 3h after the last dose treatment rats were sacrificed and intestinal mesentery was taken for study of mast cell. Histopathology of intestinal mesenteric tissue were also done for the confirmation of mast cell stabilizing activity. Results: In the unsensitized rats, 12.55±1.8% of the mast cells were found to be in the process of degranulation. In the sensitized untreated rats, 80.90±4.65% of the mast cells were degranulating when challenged with the antigen. At the doses used, prednisolone as reference standard were found to inhibit degranulation of mast cells to an extent of 72.25±3.91%, respectively. The ethanolic extract at 200 and 400 mg/kg body weight was found to inhibit degranulation of mast cells to an extent of 57.25±2.96 and 68.34±3.50%, respectively. All the values obtained were highly significant (P < 0.001,Table 1). Conclusions:The effect of ethanolic extract at two different dose level (200mg/kg and 400mg/kg), markedly protected the rats against antigen induced challenge of mast cell. Preliminary phytochemical analysis showed positive response for flavonoids. Flavonoids are reported as mast cell stabilizer active principle of plant origin. Sponsored Research - None Table 1-Mast cell stabilizing activity of ethanolic extract of fruits of Piper longum. *Not treated with horse serum and triple antigen. Values are mean ± S.E. n=6 *P < 0.001(Students t-test)